Environmental conditions encountered in forensic casework such as mass disasters and missing persons scenarios can lead to severe degradation of human DNA samples. Such highly degraded samples often produce incomplete or no STR profiles and compromises the integrity of forensic analysis. The increase in CODIS core loci from 13 to 20 in the U.S. aimed to reduce the potential for adventitious matches occurring within the database, to increase international compatibility for data sharing, and to increase discrimination power in missing persons cases. Although manufacturers introduced 6-dye multiplexes to accommodate the additional loci, it became clear that the multiplexes had limited space for these loci and any future loci additions would not be ideal due to space limitations in each channel. Promega's PowerPlex 35GY System is the first 8-dye STR multiplex, and allows co-amplification and fluorescent detection of the 20 CODIS core loci plus Amelogenin and DYS391 for sex determination. Download this infographic now to learn how this system provides improved and more informative analyses for even the most challenging DNA samples.
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