Comparison and optimization of two qPCR DNA quantification assays to improve STR analysis workflow efficiency

May 31, 2022

Analysts in forensic labs routinely perform DNA profiling analyses, including STR analyses, as part of their casework investigations. In many labs, including the Forensic Science Laboratory of the French Gendarmerie (IRCGN), the STR analysis workflow consists of DNA extraction and purification, followed by STR amplification, for all samples that are received. In cases where amplification fails, an appropriate rework strategy must be determined based on the resulting STR profile data. This review is time-consuming and reduces the efficiency and throughput of DNA labs. However, forensic labs can save time and resources by performing a DNA quantification step between the purification and STR amplification. This quick additional step allows analysts to pre-select which samples to amplify and optimize the PCR amplification with a normalized amount of input DNA.

 

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