Massively Parallel Sequencing and STR Typing: Basics and Forensic Applications
Now Available On DemandMassively parallel sequencing (MPS), which has been described as next generation sequencing, has advanced substantially and offers the forensic scientific community novel and enhanced approaches to DNA typing. While capillary electrophoresis-based technologies have been the standard method for human identity typing applications, there are limitations in this methodology’s resolution, scalability, and throughput. MPS has the potential to address these limitations and expand investigative capabilities and typing success. Because of it, high throughput MPS provides the ability to multiplex multiple types of forensically relevant genetic markers and sequence multiple samples per analysis. Additionally, MPS data may improve interpretation of mixtures.This webinar will present:     an overview of currently available MPS systems     a review of the basic terminology     general sample preparation (i.e., PCR and library) and sequencing chemistries     an illustration of how they can fit into a laboratory workflow that enables high-throughput and fast run times at a reasonable cost Technology tools that can meet forensic science needs and requirements for implementation are becoming available. A prototype NGS STR Multiplex System was used with Illumina's TruSeq™ DNA LT Sample Preparation Kit and run on the MiSeq system. Results from these studies will be shared demonstrating that this STR multiplex system, can generate reliable STR profiles at a sensitivity level that competes with current widely used CE-based methods. Speaker:Dr. Bruce Budowle, PhDExecutive Director, Institute of Applied GeneticsUniversity of North Texas