 Improved DNA Analysis Through Real-Time PCR Analysis
Multiplex STR analysis has long been accepted as the gold standard in the field of human identification. This method is highly informative, allowing DNA identification to be made with a high degree of accuracy. However, many variables can affect the quality and success of an STR analysis.
To provide optimal results, STR assays require a defined range of template material. The most commonly available STR megaplexes for forensic analysis have been optimized for template input of 0.5-1.5ng of DNA. Using template quantities less than the suggested range increases the likelihood of allelic imbalance, and partial amplification. Conversely, too much template can lead to signal saturation, nonspecific amplification, and imbalance between loci. Accurate quantitation of template DNA is necessary for optimal STR results, while conserving limited DNA material. This reduces the need for re-amplification and corresponding costs associated with additional analyses.
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